MiRduplexSVM - miRNA:miRNA* Duplex Evaluator.

It evaluates a miRNA:miRNA* duplex by assigning MiRduplexSVM's score. 
-  You can upload three  txt  files  with your sequences in fasta format, (include fasta 
file header).  The first  file should have the miRNA precursor sequences,  the 
second should include the  mature miRNA sequences  produced by the 5' strand 
and the third file the mature miRNA sequences produced by the 3' strand.

Output is a four  columns tab delimited table. The first column specifies the sequences' 
IDs as they were specified by the user, the second and the third columns show the 
matures from the 5' and the 3' strand  of the hairpin respectively, the fourth column 
represents MiRduplexSVM's score. 

Entries for which the RNAfold output is not a hairpin or consists of multiple stems are 
filtered out and 'Multibranch -  No prediction' is returned as an output at the respective 
line.
An e-mail could be provided in order to receive your results.

Thank you for using MiRduplexSVM - miRNA:miRNA* Duplex Evaluator


Input example - precursors sequences:
>MI0000063
CGGGGUGAGGUAGUAGGUUGUGUGGUUUCAGGGCAGUGAUGUUGCCCCUCGGAAGAUAACU
AUACAACCUACUGCCUUCCCUG
>MI0000286
ACCCGGCAGUGCCUCCAGGCGCAGGGCAGCCCCUGCCCACCGCACACUGCGCUGCCCCAGACC
CACUGUGCGUGUGACAGCGGCUGAUCUGUGCCUGGGCAGCGCGACCC
>MI0000447
UGAGCUGUUGGAUUCGGGGCCGUAGCACUGUCUGAGAGGUUUACAUUUCUCACAGUGAACC
GGUCUCUUUUUCAGCUGCUUC
>MI0000450
ACAAUGCUUUGCUAGAGCUGGUAAAAUGGAACCAAAUCGCCUCUUCAAUGGAUUUGGUCCC
CUUCAACCAGCUGUAGCUAUGCAUUGA
>MI0000451
GGGAGCCAAAUGCUUUGCUAGAGCUGGUAAAAUGGAACCAAAUCGACUGUCCAAUGGAUUU
GGUCCCCUUCAACCAGCUGUAGCUGUGCAUUGAUGGCGCCG
>MI0000474
CAGGGUGUGUGACUGGUUGACCAGAGGGGCAUGCACUGUGUUCACCCUGUGGGCCACCUAG
UCACCAACCCUC


Input example - mature sequences from 5'strand :
>MI0000063 
UGAGGUAGUAGGUUGUGUGGUU
>MI0000286
AGCCCCUGCCCACCGCACACUG
>MI0000447
CGGGGCCGUAGCACUGUCUGAGA
>MI0000450
AGCUGGUAAAAUGGAACCAAAU
>MI0000451
AGCUGGUAAAAUGGAACCAAAU
>MI0000474
UGUGACUGGUUGACCAGAGGGG


Input example - mature sequences from 3'strand :
>MI0000063
CUAUACAACCUACUGCCUUCCC
>MI0000286
CUGUGCGUGUGACAGCGGCUGA
>MI0000447
UCACAGUGAACCGGUCUCUUU
>MI0000450
UUUGGUCCCCUUCAACCAGCUG
>MI0000451
UUUGGUCCCCUUCAACCAGCUG
>MI0000474
CCUGUGGGCCACCUAGUCACCAA


Output example:
sequenceID 5strandMature 3strandMature MiRduplexSVM-score
MI0000063 Multibranch - No prediction
MI0000286 AGCCCCUGCCCACCGCACACUG  CUGUGCGUGUGACAGCGGCUGA  1.175949e+00
MI0000447 CGGGGCCGUAGCACUGUCUGAGA UCACAGUGAACCGGUCUCUUU  1.003558e+00
MI0000450 AGCUGGUAAAAUGGAACCAAAU  UUUGGUCCCCUUCAACCAGCUG  1.380121e+00
MI0000451 AGCUGGUAAAAUGGAACCAAAU  UUUGGUCCCCUUCAACCAGCUG  1.121841e+00
MI0000474 UGUGACUGGUUGACCAGAGGGG  CCUGUGGGCCACCUAGUCACCAA -6.557550e-02